Recombinant humaan ZCCHC17 eiwit

ZCCHC17, also known as pNO40 or PS1D, is a 241 amino acid protein that interacts with both Pinin and the 60S ribosomal subunit. Localizing to nucleolus, ZCCHC17 is ubiquitously expressed and has been suggested to play a role in ribosome maturation and biogenesis. Recombinant human ZCCHC17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleolar protein of 40 kDa, HSPC251, pNO40, PS1D, RP11-266K22.1, zinc finger CCHC domain containing 17, putative S1 RNA-binding domain protein

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30 kDa (264aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ZCCHC17, Nucleolar protein of 40 kDa, HSPC251, pNO40, PS1D, RP11-266K22.1, zinc finger CCHC domain containing 17, putative S1 RNA-binding domain protein, ATGP0977-10 µg, ATGP0977-20 µg, ATGP0977-50 µg, ATGP0977-100 µg, ATGP0977-250 µg, ATGP0977-500 µg, ATGP0977-1 mg, ATGP0977-10, ATGP0977-20, ATGP0977-50, ATGP0977-100, ATGP0977-250, ATGP0977-500, ATGP0977-1

Chang W.-L., et al. (2003) Biochem. Biophys. Res. Commun. 307:569-577; ; Betarbet, R., et al. (2008) Neurobiol. Dis. 31: 309-315

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.1M NaCl, 0.1mM PMSF

Metabolism

NP_057589

Q9NP64

Human

MNSGRPE TMENLPALYT IFQGEVAMVT DYGAFIKIPG CRKQGLVHRT HMSSCRVDKP SEIVDVGDKV WVKLIGREMK NDRIKVSLSM KVVNQGTGKD LDPNNVIIEQ EERRRRSFQD YTGQKITLEA VLNTTCKKCG CKGHFAKDCF MQPGGTKYSL IPDEEEEKEE AKSAEFEKPD PTRNPSRKRK KEKKKKKHRD RKSSDSDSSD SESDTGKRAR HTSKDSKAAK KKKKKKKHKK KHKE