Recombinant humaan ZFAND1-eiwit

AN1-type zinc finger protein 1 isoform a, also known as ZFAND1, belongs to the Zinc-finger proteins. Zinc -finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND1 is a 268 amino acid protein that contains 2 AN1-type zinc fingers, which are often found in proteins that contain an ubiquitin-like domain and therefore play a role in the ubiquitination pathway. ZFAND1 contains six conserved cysteines and two histidines and have a dimetal (zinc) -bound alpha/beta fold. There are two isoforms of ZFAND1 that are produced as a result of alternative splicing events. Recombinant human ZFAND1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

AN1-type zinc finger protein 1 isoform a, AN1-type zinc finger protein 1 isoform a, zinc finger, AN1-type domain

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.2 kDa (291aa) confirmed by MALDI-TOF

ZFAND1, AN1-type zinc finger protein 1 isoform a, AN1-type zinc finger protein 1 isoform a, zinc finger, AN1-type domain, ATGP2078-10 µg, ATGP2078-20 µg, ATGP2078-50 µg, ATGP2078-100 µg, ATGP2078-250 µg, ATGP2078-500 µg, ATGP2078-1 mg, ATGP2078-10, ATGP2078-20, ATGP2078-50, ATGP2078-100, ATGP2078-250, ATGP2078-500, ATGP2078-1

Laity J H., et al. (2001) Curr Opin Struct Biol. 11:39-46; ; Matthews J M., et al. (2002) IuBMB Life. 54:351-355.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Other

NP_078975

Q8TCF1

Human

MGSSHHHHHH SSGLVPRGSH MGSMAELDIG QHCQVEHCRQ RDFLPFVCDD CSGIFCLEHR SRESHGCPEV TVINERLKTD QHTSYPCSFK DCAERELVAV ICPYCEKNFC LRHRHQSDHE CEKLEIPKPR MAATQKLVKD IIDSKTGETA SKRWKGAKNS ETAAKVALMK LKMHADGDKS LPQTERIYFQ VFLPKGSKEK SKPMFFCHRW SIGKAIDFAA SLARLKNDNN KFTAKKLRLC HITSGEALPL DHTLETWIAK EDCPLYNGGN IILEYLNDEE QFCKNVESYL E