Recombinant humaan ZFAND3-eiwit

ZFAND3, also known as AN1-type zinc finger protein 3, contains DNA-binding domain and has a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND3 is a 251 amino acid protein containing two AN1-type zinc fingers and two uIM (ubiquitin-interacting motif) repeats. Conserved in animals and plants, the AN1-type zinc finger domain is often found in proteins that contain a ubiquitin-like domain, which suggests a role in the ubiquitination pathway. Recombinant human ZFAND3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

AN1-type zinc finger protein 3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.7 kDa (251aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ZFAND3, AN1-type zinc finger protein 3, ATGP1385-10 µg, ATGP1385-20 µg, ATGP1385-50 µg, ATGP1385-100 µg, ATGP1385-250 µg, ATGP1385-500 µg, ATGP1385-1 mg, ATGP1385-10, ATGP1385-20, ATGP1385-50, ATGP1385-100, ATGP1385-250, ATGP1385-500, ATGP1385-1

Klug A. et al. (1999) J. Mol. Biol. 293: 215-218.; ; Laity J.H. et al. (2007) Curr. Opin. Struct. Biol. 11: 39-46.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_068762

Q9H8U3

Human

MGSSHHHHHH SSGLVPRGSH MGSHMGDAGS ERSKAPSLPP RCPCGFWGSS KTMNLCSKCF ADFQKKQPDD DSAPSTSNSQ SDLFSEETTS DNNNTSITTP TLSPSQQPLP TELNVTSPSK EECGPCTDTA HVSLITPTKR SCGTDSQSEN EASPVKRPRL LENTERSEET SRSKQKSRRR CFQCQTKLEL VQQELGSCRC GYVFCMLHRL PEQHDCTFDH MGRGREEAIM KMVKLDRKVG RSCQRIGEGC S