Recombinant humaan ZFAND5 eiwit

ZFAND5 is involved in protein degradation via the ubiquitin-proteasome system. This protein may act by anchoring ubiquitinated proteins to the proteasome and plays a role in ubiquitin-mediated protein degradation during muscle atrophy. ZFAND5 plays a role in the regulation of NF-kappa-B activation and apoptosis and inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. It inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Recombinant human ZFAND5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

AN1-type zinc finger protein 5, AN1-type zinc finger protein 5, ZA20D2, ZFAND5A, ZNF216

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ZFAND5, AN1-type zinc finger protein 5, AN1-type zinc finger protein 5, ZA20D2, ZFAND5A, ZNF216, ATGP2931-10 µg, ATGP2931-20 µg, ATGP2931-50 µg, ATGP2931-100 µg, ATGP2931-250 µg, ATGP2931-500 µg, ATGP2931-1 mg, ATGP2931-10, ATGP2931-20, ATGP2931-50, ATGP2931-100, ATGP2931-250, ATGP2931-500, ATGP2931-1

Huang J., et al. (2004) J. Biol. Chem. 279:16847-16853.; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid In. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Apoptosis

NP_005998

O76080

Human

MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP YDYKAEAAAK IRKENPVVVA EKIQRI