Recombinant humaan ZMAT3-eiwit

ZMAT3 is a protein containing three zinc finger domains and a nuclear localization signal. The mRNA and the protein of this gene are upregulated by wildtype p53 and overexpression of this gene inhibits tumor cell growth, suggesting that this gene may have a role in the p53-dependent growth regulatory pathway. Alternative splicing of this gene results in two transcript variants encoding two isoforms differing in only one amino acid. Recombinant human ZMAT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Zinc finger matrin-type protein 3, PAG608, WIG-1, WIG1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.4 kDa (312aa) confirmed by MALDI-TOF

ZMAT3, Zinc finger matrin-type protein 3, PAG608, WIG-1, WIG1, ATGP2252-10 µg, ATGP2252-20 µg, ATGP2252-50 µg, ATGP2252-100 µg, ATGP2252-250 µg, ATGP2252-500 µg, ATGP2252-1 mg, ATGP2252-10, ATGP2252-20, ATGP2252-50, ATGP2252-100, ATGP2252-250, ATGP2252-500, ATGP2252-1

Hellborg F., et al. (2001) Oncogene. 20:5466-5474; ; Mendez Vidal C., et al. (2006) FEBS Lett. 580:4401-4408

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Metabolism

NP_071915.1

Q9HA38

Human

MILLQHA VLPPPKQPSP SPPMSVATRS TGTLQLPPQK PFGQEASLPL AGEEELSKGG EQDCALEELC KPLYCKLCNV TLNSAQQAQA HYQGKNHGKK LRNYYAANSC PPPARMSNVV EPAATPVVPV PPQMGSFKPG GRVILATEND YCKLCDASFS SPAVAQAHYQ GKNHAKRLRL AEAQSNSFSE SSELGQRRAR KEGNEFKMMP NRRNMYTVQN NSAGPYFNPR SRQRIPRDLA MCVTPSGQFY CSMCNVGAGE EMEFRQHLES KQHKSKVSEQ RYRNEMENLG YV