Recombinant humaan ZWINT-eiwit

ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts's syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Recombinant human ZWINT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ZW10 interactor isoform b, HZwint-1, KNTC2AP, ZWINT1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.9 kDa (253aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ZWINT, ZW10 interactor isoform b, HZwint-1, KNTC2AP, ZWINT1, ATGP2646-10 µg, ATGP2646-20 µg, ATGP2646-50 µg, ATGP2646-100 µg, ATGP2646-250 µg, ATGP2646-500 µg, ATGP2646-1 mg, ATGP2646-10, ATGP2646-20, ATGP2646-50, ATGP2646-100, ATGP2646-250, ATGP2646-500, ATGP2646-1

Musio A., et al. (2004) Gene. 331: 33-40.; ; Lin Y T., et al. (2006) Oncogene. 25: 6901-6914.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Cell Cycle

NP_001005413

O95229

Human

MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP