Recombinant humaanCarbonisch Anhydrase 13/CA13 eiwit

CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon) . These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonic anhydrase 13, CAXIII, Carbonic anhydrase XIII, Carbonate dehydratase XIII

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

31.8 kDa (285aa) confirmed by MALDI-TOF

CAXIII, CA 13, CA XIII, CA13, Carbonate dehydratase 13, Carbonate dehydratase XIII, Carbonate dehydratase13, Carbonate dehydrataseXIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, Carbonic anhydrase13, Carbonic anhydraseXIII, ATGP3059-10 µg, ATGP3059-20 µg, ATGP3059-50 µg, ATGP3059-100 µg, ATGP3059-250 µg, ATGP3059-500 µg, ATGP3059-1 mg, ATGP3059-010, ATGP3059-020, ATGP3059-050, ATGP3059-100, ATGP3059-250, ATGP3059-500, ATGP3059-01M

Lamesch P., et al. (2007) Genomics 89 (3), 307-315

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_940986.1

Q8N1Q1

Human

MSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH