Recombinant Human Rho Family GTPase 3

Source : Escherichia Coli. RND3 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-241 a.a.) and having a molecular mass of 29.2 kDa. The RND3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. RND3 is a small GTPase which is part of the Ras superfamily that controls the association of the actin cytoskeleton in reaction to extracellular growth factors. RND3 activity causes a decrease in stress fibers and an increase in cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. RND3 inhibits DNA-damage induced apoptosis and cell cycle arrest. RND3 binds GTP and is resistant to Rho-specific GTPase-activating proteins.

ARHE||memB||Rho8||RhoE||Rho-related GTP-binding protein RhoE||Protein MemB||Rho family GTPase 3||Rho-related GTP-binding protein Rho8||Rnd3.

Greater than 90.0% as determined by SDS-PAGE.

RND3 Human solution containing 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA, 0.1M NaCl & 40% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

MGSSHHHHHH SSGLVPRGSH MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C.