Recombinant Influenza A H3N2 Hemagglutinine/HA1-eiwit

Influenza A virus H3N2 is a subtype of viruses that cause influenza (flu) . H3N2 viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Recombinant Influenza A virus (A/Perth/16/2009 H3N2) HA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Hemagglutinin, Influenza A virus (A/Perth/16/2009 (H3N2) ) hemagglutinin, HA

Baculovirus

Human, Influenza A-H3N2 (human)

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

37.8 kDa (339aa)

Hemagglutinin, ATGP1481-10 µg, ATGP1481-20 µg, ATGP1481-50 µg, ATGP1481-100 µg, ATGP1481-250 µg, ATGP1481-500 µg, ATGP1481-1 mg, ATGP1481-10, ATGP1481-20, ATGP1481-50, ATGP1481-100, ATGP1481-250, ATGP1481-500, ATGP1481-1

Alymova IV., et al. (2011) J Virol. 85 (23) :12324-33.; ; Cappuccio JA., et al. (2011) J Gen Virol. 92 (12) :2871-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Other

ACS71642.1

C6KNH7

Human, Influenza A-H3N2 (human)

QKLPGN DNSTATLCLG HHAVPNGTIV KTITNDQIEV TNATELVQSS STGEICDSPH QILDGKNCTL IDALLGDPQC DGFQNKKWDL FVERSKAYSN CYPYDVPDYA SLRSLVASSG TLEFNNESFN WTGVTQNGTS SACIRRSKNS FFSRLNWLTH LNFKYPALNV TMPNNEQFDK LYIWGVHHPG TDKDQIFLYA QASGRITVST KRSQQTVSPN IGSRPRVRNI PSRISIYWTI VKPGDILLIN STGNLIAPRG YFKIRSGKSS IMRSDAPIGK CNSECITPNG SIPNDKPFQN VNRITYGACP RYVKQNTLKL ATGMRNVPEK QTR