Recombinant Influenza A H3N2 Hemagglutinine/HA1-eiwit

Influenza A virus subtype H3N2, also known as H3N2, is a subtype of viruses that cause influenza (flu) . H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, HA, Influenza A virus (A/canine/Guangdong/1/2006 (H3N2) )

Baculovirus

Canine, Influenza A-H3N2 (canine), Influenza A

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

36.9 kDa (336aa)

Hemagglutinin, ATGP1488-10 µg, ATGP1488-20 µg, ATGP1488-50 µg, ATGP1488-100 µg, ATGP1488-250 µg, ATGP1488-500 µg, ATGP1488-1 mg, ATGP1488-10, ATGP1488-20, ATGP1488-50, ATGP1488-100, ATGP1488-250, ATGP1488-500, ATGP1488-1

Li S., et al. (2010) Infect. Genet. Evol. 10:1286-1288; ; Song D., et al. (2008) Emerg. Infect. Dis. 14:741-746

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Other

ADB45177.1

E2E3B0

Canine, Influenza A-H3N2 (canine), Influenza A

Ala

NLPGNENN AATLCLGHHA VPNGTIVKTI TDDQIEVTNA TELVQNSSTG KICNNPHKIL DGRDCTLIDA LLGDPHCDVF QNETWDLFVE RSNAFSNCYP YDVPDYASLR SIVASSGTLE FITEGFTWAG VTQNGGSGAC KKGPANGFFS RLNWLTKSGN TYPVLNVTMP NNNNFDKLYI WGVHHPSTNQ EQTSLYIQAS GRVKVSTRRS QQTIIPNIGS RPLVRGQSGR ISVYWTIVKP GDVLVINSNG NLIAPRGYFK MRIGKSSIMR SDAPIDTCIS ECITPNGSIP NEKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPERQT