Recombinant Influenza A H5N1 Hemagglutinine/HA1-eiwit

H5N1/HA (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/Viet Nam/HN31242/2007 (H5N1) ) HA1 protein, fused to His-tag at C-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

Hemagglutinin, Influenza A virus (A/Vietnam/HN31242/2007H5N1) hemagglutinin, H, HA

Baculovirus

Influenza A-H5N1

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

37.4 kDa (332aa)

Hemagglutinin, ATGP1497-10 µg, ATGP1497-20 µg, ATGP1497-50 µg, ATGP1497-100 µg, ATGP1497-250 µg, ATGP1497-500 µg, ATGP1497-1 mg, ATGP1497-10, ATGP1497-20, ATGP1497-50, ATGP1497-100, ATGP1497-250, ATGP1497-500, ATGP1497-1

Li S., et al. (2010) Infect. Genet. Evol. 10:1286-1288; ; Song D., et al. (2008) Emerg. Infect. Dis. 14:741-746

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Other

ABY19417

A9YU04

Influenza A-H5N1

DQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPL