Recombinant M. tuberculose Antigen 85A eiwit

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C) . The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6, 6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A, Ag85A, DGAT, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, Acyl-CoA diacylglycerol acyltransferase, fbpA, Rv3804c

Baculovirus

Mycobacterium tuberculosis

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

32.8 kDa (305aa)

Antigen85A, Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A, ATGP1490-10 µg, ATGP1490-20 µg, ATGP1490-50 µg, ATGP1490-100 µg, ATGP1490-250 µg, ATGP1490-500 µg, ATGP1490-1 mg, ATGP1490-10, ATGP1490-20, ATGP1490-50, ATGP1490-100, ATGP1490-250, ATGP1490-500, ATGP1490-1

Elamin AA., et al. (2009) J Microbiol Methods. 79 (3) :358-63.; ; Jeon BY., et al. (2011) Microbes Infect. 13 (3) :284-90.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Other

NP_218321.1

P9WQP3

Mycobacterium tuberculosis

FSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGA