Recombinant M. tuberculose ESAT6-eiwit

ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Early secretory antigenic target 6 kDa, esxA, esaT6

Baculovirus

Mycobacterium tuberculosis

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

11 kDa (104aa)

ESAT6, ATGP1480-10 µg, ATGP1480-20 µg, ATGP1480-50 µg, ATGP1480-100 µg, ATGP1480-250 µg, ATGP1480-500 µg, ATGP1480-1 mg, ATGP1480-10, ATGP1480-20, ATGP1480-50, ATGP1480-100, ATGP1480-250, ATGP1480-500, ATGP1480-1, Early secretory antigenic target, 6 kDa

Pathak SK., et al. (2007) Natlmmunol. 8 (6) :610-8.; ; Matveeva NA., et al. (2011) Tsitol Genet. 45 (1) :11-7.

Satake Y, et al. Type-1 polarised dendritic cells are a potent immunogen against Mycobacterium tuberculosis. (Int J Tuberc Lung Dis. 2017) {https://pubmed.ncbi.nlm.nih.gov/28399967/}; ; Ouyang W, et al. Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization. (Lab Chip. 2017) {https://pubmed.ncbi.nlm.nih.gov/28983543/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Other

NP_338543.1

P9WNK6

Mycobacterium tuberculosis

MTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFA