Recombinant Mouse Erytropoëtine/EPO Proteïne

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant mouse EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.

Erythropoietin, erythropoietin isoform 1 precursor, Epo

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.

19.8kDa (176aa)

, ATGP3959-10 µg, ATGP3959-20 µg, ATGP3959-50 µg, ATGP3959-100 µg, ATGP3959-250 µg, ATGP3959-500 µg, ATGP3959-1 mg, ATGP3959-10, ATGP3959-20, ATGP3959-50, ATGP3959-100, ATGP3959-250, ATGP3959-500, ATGP3959-1

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Angiogenesis, Cardiovascular

NP_031968

P07321

Mouse

Ala

MAPPRLI CDSRVLERYI LEAKEAENVT MGCAEGPRLS ENITVPDTKV NFYAWKRMEV EEQAIEVWQG LSLLSEAILQ AQALLANSSQ PPETLQLHID KAISGLRSLT SLLRVLGAQK ELMSPPDTTP PAPLRTLTVD TFCKLFRVYA NFLRGKLKLY TGEVCRRGDR