Recombinant MSLN Antilichaam / Mesotheline

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

Q13421

Human

A portion of amino acids 250-450 from human Mesothelin protein was used as the immunogen for the recombinant MSLN antibody.

MSLN/8331R

IHC-P, WB

Protein A affinity

Purified

This recombinant MSLN antibody is available for research use only.

Aliquot the recombinant MSLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant MSLN antibody should be determined by the researcher.

Cell surface, Secreted

IHC staining of FFPE human ovarian carcinoma tissue with recombinant MSLN antibody (clone MSLN/8331R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.