Recombinant muis 15-PGDH/HPGD eiwit

Hpgd, also known as Hydroxyprostaglandin dehydrogenase, is a member of the short-chain dehydrogenases/reductases family. It metabolizes prostaglandins (PGs) to render them. Prostaglandins (PGs) play a crucial role in mediating parturition events, and their synthesis and metabolism are regulated by PGH synthase and 15-hydroxy-PG dehydrogenase (PGDH), respectively. Hpgd, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers. Recombinant mouse Hpgd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

15-hydroxyprostaglandin dehydrogenase [NAD+], 15-PGDH, PGDH, PGDH1, SDR36C1, Prostaglandin dehydrogenase 1

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

31.6 kDa (292aa)

15 Hydroxyprostaglandin dehydrogenase, 15 Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-Hydroxyprostaglandin dehydrogenase, 15-Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, 15-PGDH, AV026552, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1, ATGP3517-10 µg, ATGP3517-20 µg, ATGP3517-50 µg, ATGP3517-100 µg, ATGP3517-250 µg, ATGP3517-500 µg, ATGP3517-1 mg, ATGP3517-010, ATGP3517-020, ATGP3517-050, ATGP3517-100, ATGP3517-250, ATGP3517-500, ATGP3517-01M

Patel FA. et al., (2003) J Clin Endocrinol Metab. 88 (6) :2922-33.; ; McKeown KJ. et al., (2003) J Clin Endocrinol Metab. 88 (4) :1737-41.; ; Yan M. et al., (2004) Proc Natl Acad Sci USA. 101 (50) :17468-73.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol

Signal Transduction

NP_032304.2

Q8VCC1

Mouse

MHVNGKV ALVTGAAQGI GKAFAEALLL HGAKVALVDW NLEAGVKCKA ALDEQFEPQK TLFVQCDVAD QKQLRDTFRK VVDHFGRLDI LVNNAGVNNE KNWEQTLQIN LVSVISGTYL GLDYMSKQNG GEGGIIINMS SLAGLMPVAQ QPVYCASKHG IIGFTRSAAM AANLMKSGVR LNVICPGFVD TPILESIEKE ENMGQYIEYK DQIKAMMKFY GVLHPSTIAN GLINLIEDDA LNGAIMKITA SKGIHFQDYD ISPLLVKAPL TS