Recombinant muis 5′-Nucleotidase/CD73 eiwit

Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of general functional importance for the metabolism of nucleotides at the ventricular surface of the retina as well as the ventricles of the brain, a feature that is maintained throughout development. Recombinant mouse Nt5e, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 8,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

59.1 kDa (531aa)

Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ATGP3509-10 µg, ATGP3509-20 µg, ATGP3509-50 µg, ATGP3509-100 µg, ATGP3509-250 µg, ATGP3509-500 µg, ATGP3509-1 mg, ATGP3509-010, ATGP3509-020, ATGP3509-050, ATGP3509-100, ATGP3509-250, ATGP3509-500, ATGP3509-01M

Koszalka P., et al. (2016) PLoS One. 11:e0151420.; ; Braun N., et al. (1995) Brain Res Dev Brain Res. 88:79-86.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_035981.1

Q61503

Mouse

Trp29