Recombinant muis ACE-2 eiwit

ACE-2, also known as angiotensin-converting enzyme 2, is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. It cleaves angiotensin 1 to angiotensin 1-9 and angiotensin 2 to angiotensin 1-7 as a carboxypeptidase. It may be an important regulator of heart function and have a protective role in acute lung injury. Also, it is a key SARS-CoV Spike protein receptor in vivo. Recombinant mouse ACE-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Processed Angiotensin-converting enzyme 2, ACE2, 2010305L05Rik

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 200 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK (Dnp) -OH per minute at pH 7.5, at 25C.

84.5 kDa (731aa)

ATGP3288, ATGP4014-10 µg, ATGP4014-20 µg, ATGP4014-50 µg, ATGP4014-100 µg, ATGP4014-250 µg, ATGP4014-500 µg, ATGP4014-1 mg, ATGP4014-10, ATGP4014-20, ATGP4014-50, ATGP4014-100, ATGP4014-250, ATGP4014-500, ATGP4014-1, ATGP4014-10 µg, ATGP4014-20 µg, ATGP4014-50 µg, ATGP4014-100 µg, ATGP4014-250 µg, ATGP4014-500 µg, ATGP4014-1 mg, ATGP4014-10, ATGP4014-20, ATGP4014-50, ATGP4014-100, ATGP4014-250, ATGP4014-500, ATGP4014-1

Kuba K., et al. (2005) Nature Med. 11:875-879.; ; Ima Y., et al. (2005) Nature 436:112-116.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_081562.2

Q8R0I0

Mouse

Gln18