Recombinant muis Acy1-eiwit

Acy1 also known as Aminoacylase1. The protein is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21. 1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. Recombinant mouse Acy1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aminoacylase 1, ACY-1, Acy1, ACY1_HUMAN, ACY1D, ACYLASE, Acylase I, Aminoacylase-1, EC 3.5.1.14, epididymis secretory protein Li 5, HEL-S-5, N acyl L amino acid amidohydrolase, N-acyl-L-amino-acid amidohydrolase, OTTHUMP00000212459, OTTHUMP00000212462, OTTHUMP00000212463, OTTHUMP00000212464, OTTHUMP00000212465

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

48.4 kDa (433aa) confirmed by MALDI-TOF

Acy1, Aminoacylase 1, ACY-1, Acy1 , ACY1_HUMAN , ACY1D , ACYLASE , Acylase I , Aminoacylase-1 , EC 3.5.1.14 , epididymis secretory protein Li 5 , HEL-S-5 , N acyl L amino acid amidohydrolase , N-acyl-L-amino-acid amidohydrolase , OTTHUMP00000212459 , OTTHUMP00000212462 , OTTHUMP00000212463 , OTTHUMP00000212464 , OTTHUMP00000212465, ATGP3131-10 µg, ATGP3131-20 µg, ATGP3131-50 µg, ATGP3131-100 µg, ATGP3131-250 µg, ATGP3131-500 µg, ATGP3131-1 mg, ATGP3131-10, ATGP3131-20, ATGP3131-50, ATGP3131-100, ATGP3131-250, ATGP3131-500, ATGP3131-1

Miller YE, Drabkin H, et al. (1991) Genomics 8 (1) : 149-154. ; ; Voss R, et al. (1982) Ann Hum Genet 44 (Pt 1) : 1-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

AAH05631

Q99JW2

Mouse

MGSSHHHHHH SSGLVPRGSH MGSEFMTTKD PESEHPSVTL FRQYLRICTV QPNPDYGGAI TFLEERARQL GLSCQKIEVV PGFVITVLTW PGTNPSLPSI LLNSHTDVVP VFKEHWHHDP FEAFKDSEGY IYARGSQDMK SVSIQYLEAV RRLKSEGHRF PRTIHMTFVP DEEVGGHKGM ELFVKRPEFQ ALRAGFALDE GLANPTDAFT VFYSERSPWW VRVTSTGKPG HASRFIEDTA AEKLHKVISS ILAFREKERQ RLQANPHLKE GAVTSVNLTK LEGGVAYNVV PATMSASFDF RVAPDVDMKA FEKQLQRWCQ EAGEGVTFEF AQKFTEPRMT PTDDSDPWWA AFSGACKAMN LTLEPEIFPA ATDSRYIRAV GIPALGFSPM NRTPVLLHDH NERLHEDIFL RGVDIYTGLL SALASVPTLP GES