Recombinant muis Adenosine Kinase/ADK-eiwit

Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

ADK, AK, adenosine kinase, Adenosine 5'-phosphotransferase

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 100pmol/min/ and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

42.5 kDa (384aa) confirmed by MALDI-TOF

Adk, Adenosine kinase, 2310026J05Rik, 5033405D03Rik, AI255373, AI987814, Ak, ATGP3692-10 µg, ATGP3692-20 µg, ATGP3692-50 µg, ATGP3692-100 µg, ATGP3692-250 µg, ATGP3692-500 µg, ATGP3692-1 mg, ATGP3692-10, ATGP3692-20, ATGP3692-50, ATGP3692-100, ATGP3692-250, ATGP3692-500, ATGP3692-1

Chakraborty A., et al. (2002) J Biol Chem. 277 (49: 47451-60.; ; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3) :745-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 50 mM NaCl, 1mM EDTA

Enzymes & Proteases

NP_598840

P55264

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH