Recombinant muis Adenosylhomocysteinase/AHCY-eiwit

Ahcy, also known as Adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-sdenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy) . AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant mouse Ahcy, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

S-adenosylhomocysteine hydrolase, AdoHcyase, CUBP, Liver copper-binding protein, S-adenosyl-L-homocysteine hydrolase, SAHH

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

50.2 kDa (456aa) confirmed by MALDI-TOF

Ahcy, Adenosylhomocysteinase, AA987153, AL024110, CuBP, SAHH, ATGP3650-10 µg, ATGP3650-20 µg, ATGP3650-50 µg, ATGP3650-100 µg, ATGP3650-250 µg, ATGP3650-500 µg, ATGP3650-1 mg, ATGP3650-10, ATGP3650-20, ATGP3650-50, ATGP3650-100, ATGP3650-250, ATGP3650-500, ATGP3650-1

Vugrek O., et al. (2009) Hum Mutat. 30 (4) : E555-65.; ; Park SJ. et al., (2015) Am J Cancer Res. 5 (7) :2127-2138

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT

Enzymes & Proteases

NP_057870

P50247

Mouse

MSDKLP YKVADIGLAA WGRKALDIAE NEMPGLMRMR EMYSASKPLK GARIAGCLHM TVETAVLIET LVALGAEVRW SSCNIFSTQD HAAAAIAKAG IPVFAWKGET DEEYLWCIEQ TLHFKDGPLN MILDDGGDLT NLIHTKYPQL LSGIRGISEE TTTGVHNLYK MMSNGILKVP AINVNDSVTK SKFDNLYGCR ESLIDGIKRA TDVMIAGKVA VVAGYGDVGK GCAQALRGFG ARVIITEIDP INALQAAMEG YEVTTMDEAC KEGNIFVTTT GCVDIILGRH FEQMKDDAIV CNIGHFDVEI DVKWLNENAV EKVNIKPQVD RYWLKNGRRI ILLAEGRLVN LGCAMGHPSF VMSNSFTNQV MAQIELWTHP DKYPVGVHFL PKKLDEAVAE AHLGKLNVKL TKLTEKQAQY LGMPINGPFK PDHYRY