Recombinant muis Adiponectrip30 eiwit

Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia. Recombinant mouse adiponectin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ADIPOQ, Acdc, Acrp30, Apm1, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipocyte-specific protein

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

27.2 kDa (251aa) confirmed by MALDI-TOF

Adiponectin, Acrp30, adipocyte complement-related protein, Acdc, GBP28, Acrp30, mAdiponectin, 18- 247aa, Mouse, His-tagged, Adiponectin, C1Q and collagen domain containing [Mus musculus], ADI0702-10 µg, ADI0702-20 µg, ADI0702-50 µg, ADI0702-100 µg, ADI0702-250 µg, ADI0702-500 µg, ADI0702-1 mg, ADI0702-10, ADI0702-20, ADI0702-50, ADI0702-100, ADI0702-250, ADI0702-500, ADI0702-1

Yokota. T. et al. (2000) Blood. 96:1723; ; Yamauchi T. et al (2002) Nature Medicine.8 (11) :1288-1295; ; Lu M. et al (2007) Mol Endocrinol. 59 (2) :207-16;

Jeon BT, et al. Adiponectin protects hippocampal neurons against kainic acid-induced excitotoxicity. (Brain Res Rev. 2009) {https://pubmed.ncbi.nlm.nih.gov/19460404/}; ; Suzuki Y, et al. Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells. (Biochem Biophys Res Commun. 2015) {https://pubmed.ncbi.nlm.nih.gov/26342800/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Hormones & Cytokines

NP_033735

Q60994

Mouse

EDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N