Recombinant muis AK1-eiwit

Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Adenylate kinase isoenzyme 1, Adenylate kinase 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase 1, Myokinase

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 150unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

25.5 kDa (233aa) confirmed by MALDI-TOF

Ak1, Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATGP3700-10 µg, ATGP3700-20 µg, ATGP3700-50 µg, ATGP3700-100 µg, ATGP3700-250 µg, ATGP3700-500 µg, ATGP3700-1 mg, ATGP3700-10, ATGP3700-20, ATGP3700-50, ATGP3700-100, ATGP3700-250, ATGP3700-500, ATGP3700-1

Dzeja PP., et al. (2007) J Biol Chem. 282 (43) :31366-72.; ; Ravera S., et al., (2015) Curr Eye Res. 32 (3) :249-57.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Metabolism

NP_067490.1

Q9R0Y5

Mouse

MGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK