Recombinant muis Aldehyde dehydrogenase 2/ALDH2-eiwit

Aldh2 also known as Aldehyde dehydrogenase, mitochondrial. Aldh2 belongs to the aldehyde dehydrogenase family which catalyzes the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant mouse ALDH2 was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

56.8 kDa (523aa)

Aldh2, Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase, mitochondrial, Ahd-5, Ahd5, Ahd 5, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM, AHD M1, ALDH E2, AHDM1, ALDHE2, ATGP3839-10 µg, ATGP3839-20 µg, ATGP3839-50 µg, ATGP3839-100 µg, ATGP3839-250 µg, ATGP3839-500 µg, ATGP3839-1 mg, ATGP3839-010, ATGP3839-020, ATGP3839-050, ATGP3839-100, ATGP3839-250, ATGP3839-500, ATGP3839-01M

Chang C., et al. (1994) Gene. 148:331-336.; ; Chen M., et al. (1994) Mol. Pharmacol. 46:88-96.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Metabolism

NP_033786

P47738

Mouse

MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEV ICQVAEGNKE DVDKAVKAAR AAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVIS YLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHE PVGVCGQIIP WNFPLLMQAW KLGPALATGN VVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGST EVGHLIQVAA GSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAK SRVVGNPFDS RTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAK EEIFGPVMQI LKFKTIEEVV GRANDSKYGL AAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGG YKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS