Recombinant muis Aldo-ketoreductase 1B1/AKR1B1-eiwit

Akr1b1, also known as Aldose reductas, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Recombinant mouse Akr1b1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Aldose reductase, Ahr-1, Ahr1, Akr1b1, Aldor1, Aldr1, ALR2, AR, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldo-keto reductase family 1 member B3, Akr1b3

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

38.1 kDa (339aa) confirmed by MALDI-TOF

Aldose reductase, Akr1b1, Aldose reductase, Ahr-1, Ahr1, Akr1b1, Aldor1, Aldr1, ALR2, AR, ATGP2982-10 µg, ATGP2982-20 µg, ATGP2982-50 µg, ATGP2982-100 µg, ATGP2982-250 µg, ATGP2982-500 µg, ATGP2982-1 mg, ATGP2982-10, ATGP2982-20, ATGP2982-50, ATGP2982-100, ATGP2982-250, ATGP2982-500, ATGP2982-1

Wilson DK, Bohren KM, et al. (1992) . Science. 257 (5066) :81-4.; ; Graham A, Brown L, et al. (1992) . J Biol Chem. 266 (11) :6872-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_033788

P45376

Mouse

MASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV