Recombinant muis-alfa-synucleïne-eiwit

Alpha-Synuclein, also known as SNCA, an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-Synuclein has been suggested to be implicated in the pathogenesis of Parkinson. Recombinant mouse alpha-Synuclein, was expressed in E. coli and purified by using conventional chromatography techniques

Alpha-Synuclein, Snca, Syn, alpha-Synuclein, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

E.coli

Mouse

Non-Tagged

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

14.4 kDa (140aa) confirmed by MALDI-TOF

A-Synuclein, alpha-Synuclein, Snca, Syn, alpha-Synuclein, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, ATGP0619-10 µg, ATGP0619-20 µg, ATGP0619-50 µg, ATGP0619-100 µg, ATGP0619-250 µg, ATGP0619-500 µg, ATGP0619-1 mg, ATGP0619-10, ATGP0619-20, ATGP0619-50, ATGP0619-100, ATGP0619-250, ATGP0619-500, ATGP0619-1

Ueda K., et al. (1993) Proc. Natl. Acad. Sci. uSA 90:11282-11286 ; ; Kim J., et al. (1997) Molecules and Cells 7:78-83 ; ; Jakes R., et al. (1994) FEBS lett. 345:27-32. ;

Kim S, et al. Alpha-synuclein induces migration of BV-2 microglial cells by up-regulation of CD44 and MT1-MMP. (J Neurochem. 2009) {https://pubmed.ncbi.nlm.nih.gov/19457162/}; ; Joo SH, et al. Regulation of matrix metalloproteinase-9 and tissue plasminogen activator activity by alpha-synuclein in rat primary glial cells. (Neurosci Lett. 2010) {https://pubmed.ncbi.nlm.nih.gov/20026244/}; ; Lee HJ, et al. Enzyme-linked immunosorbent assays for α-synuclein with species and multimeric state specificities. (J Neurosci Methods. 2011) {https://pubmed.ncbi.nlm.nih.gov/21658411/}; ; Park HJ, et al. Mesenchymal stem cells enhance α-synuclein clearance via M2 microglia polarization in experimental and human parkinsonian disorder. (Acta Neuropathol. 2016) {https://pubmed.ncbi.nlm.nih.gov/27497943/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing, 10% glycerol

Neuroscience

NP_033247

O55042

Mouse

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA