Recombinant muis ALT2/GPT2-eiwit

ALT2/GPT2, also known as alanine aminotransferase, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. Recombinant mouse ALT2/GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 50units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.

Alanine aminotransferase 2, ALT2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamate pyruvate transaminase 2

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.

60.1 kDa (542aa) confirmed by MALDI-TOF

Alanine aminotransferase2, Alanine aminotransferase 2, ALT 2, ALT2, ALT-2, Glutamate pyruvate transaminase 2, Glutamic alanine transaminase 2, Glutamic pyruvic transaminase 2, Glutamic-alanine transaminase 2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, GPT 2, GPT2, GPT-2, ATGP3881-10 µg, ATGP3881-20 µg, ATGP3881-50 µg, ATGP3881-100 µg, ATGP3881-250 µg, ATGP3881-500 µg, ATGP3881-1 mg, ATGP3881-010, ATGP3881-020, ATGP3881-050, ATGP3881-100, ATGP3881-250, ATGP3881-500, ATGP3881-01M

Sohocki M.M. et al. (1997) Genomics 40 (2) :247-52.; ; Matthews C.C. et al. (2003) Brain Res. 978 (1-2) : 59-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 2mM DTT

Metabolism

NP_776291

Q8BGT5

Mouse

QRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS