Recombinant muis Apolipoproteïne A1/APOA1-eiwit

Apoa1 also known as Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apoa1 are associated with HDL deficiency including Tangier disease. Recombinant mouse Apoa1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Apolipoprotein A-I, Alp-1, apo-AI, Apoa-1, apoA-I, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, Apolipoprotein AI

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.3 kDa (263aa) confirmed by MALDI-TOF

Apolipoprotein A-I, Apolipoprotein A-I, Alp-1, apo-AI, Apoa-1, apoA-I, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, Apolipoprotein AI, ATGP3058-10 µg, ATGP3058-20 µg, ATGP3058-50 µg, ATGP3058-100 µg, ATGP3058-250 µg, ATGP3058-500 µg, ATGP3058-1 mg, ATGP3058-10, ATGP3058-20, ATGP3058-50, ATGP3058-100, ATGP3058-250, ATGP3058-500, ATGP3058-1

Sontag TJ., et al. (2014) Arterioscler Thromb Vasc Biol, 34 (2), 262-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Cardiovascular

NP_033822.2

Q00623

Mouse

DEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ