Recombinant muis Arylsulfatase A/ARSA-eiwit

Arsa, also known as arylsulfatase A, is a prototype member of the sulfatase family. It is an enzyme that catalyzes the degradation of sulfatides, a glycosphingolipid found in many tissues, but predominantly in myelin and kidney. The serum level of its might be helpful in diagnosis of lung and central nervous system cancer. Recombinant mouse Arsa, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 4,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C

Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73, Cerebroside-sulfatase

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 4,500pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C

53.2 kDa (498aa)

Arsa, Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73, ATGP3831-10 µg, ATGP3831-20 µg, ATGP3831-50 µg, ATGP3831-100 µg, ATGP3831-250 µg, ATGP3831-500 µg, ATGP3831-1 mg, ATGP3831-10, ATGP3831-20, ATGP3831-50, ATGP3831-100, ATGP3831-250, ATGP3831-500, ATGP3831-1

Lukatela G., et al. (1998) Biochemistry. 37:3654-3664.; ; Jean S., et al. (2006) Alcohol Clin Exp Res. 30:1950-1955.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_033843

P50428

Mouse

Ala

ADPSPPNILL IFADDLGYGD LGSYGHPSST TPNLDQLAEG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRS GMYPGVLGPS SQGGLPLEEV TLAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPD IPCKGGCDQG LVPIPLLANL TVEAQPPWLP GLEARYVSFS RDLMADAQRQ GRPFFLYYAS HHTHYPQFSG QSFTKRSGRG PFGDSLMELD GAVGALMTTV GDLGLLEETL VIFTADNGPE LMRMSNGGCS GLLRCGKGTT FEGGVREPAL VYWPGHITPG VTHELASSLD LLPTLAALTG APLPNVTLDG VDISPLLLGT GKSPRKSVFF YPPYPDEIHG VFAVRNGKYK AHFFTQGSAH SDTTSDPACH AANRLTAHEP PLLYDLSQDP GENYNVLESI EGVSPEALQA LKHIQLLKAQ YDAAMTFGPS QIAKGEDPAL QICCQPSCTP HPVCCHCPGS QSHHHHHH