Recombinant muis B7-1/CD80-eiwit

Cd80, also known as T-lymphocyte activation antigen CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: Cd28 (for autoregulation and intercellular association) and Ctla4 (for attenuation of regulation and cellular disassociation) . This protein also plays a role in induction of innate immune responses by activating NF-KB-signaling pathway in macrophages. It is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. Recombinant mouse Cd80, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse CD80 is coated at 2 µg/mL (100 ul/well) can bind Mouse CTLA4 (CAT# ATGP4046) in a Functional ELISA assay.

T-lymphocyte activation antigen CD80, Cd80, Cd28l, Ly-53, Ly53, MIC17, TSA1, Activation B7-1 antigen, B7

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Mouse CTLA4 (CAT# ATGP4046) .

24.7 kDa (216aa)

B71, CD80, T-lymphocyte activation antigen CD80, Cd80, Cd28l, Ly-53, Ly53, MIC17, TSA1, ATGP3353-10 µg, ATGP3353-20 µg, ATGP3353-50 µg, ATGP3353-100 µg, ATGP3353-250 µg, ATGP3353-500 µg, ATGP3353-1 mg, ATGP3353-10, ATGP3353-20, ATGP3353-50, ATGP3353-100, ATGP3353-250, ATGP3353-500, ATGP3353-1

Peach R., et al. (1995) J. Biol. Chem. 270: 21181-21187.; ; Suvas S., et al. (2002) J. Biol. Chem. 277:7766-7775.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_033985

Q00609

Mouse

Gln37