Recombinant muis Biliverdin Reductase B/BLVRB-eiwit

Blvrb, also known as flavin reductase (NADPH) isoform 1, is an enzyme (EC 1. 3. 1. 24) that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. Blvrb is found that major erythrocytic heme catabolic pathway in humans and most mammalian species. Biliverdin reductase is abundantly expressed in kidney, spleen, liver and brain as well as at lower levels in the thymus and minimal levels being detected in testis. Recombinant mouse Blvrb protein was expressed in E. coli and purified by using conventional chromatography techniques.

Flavin reductase, NADPH, Biliverdin reductase B, BVR-B, Biliverdin-IX beta-reductase, NADPH-dependent diaphorase, NADPH-flavin reductase, FLR, FR

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

24.6 kDa (229aa) confirmed by MALDI-TOF

Blvrb, Biliverdin reductase B (flavin reductase (NADPH) ), flavin reductase (NADPH) isoform 1, ATGP3764-10 µg, ATGP3764-20 µg, ATGP3764-50 µg, ATGP3764-100 µg, ATGP3764-250 µg, ATGP3764-500 µg, ATGP3764-1 mg, ATGP3764-10, ATGP3764-20, ATGP3764-50, ATGP3764-100, ATGP3764-250, ATGP3764-500, ATGP3764-1

Baranano DE., et al. (2002) . Proc Natl Acad Sci USA. 99 (25) :16093-8.; ; Saito F., et al. (1995) Cytogenet Cell Genet. 71 (2) :179-81.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

NP_659172

Q923D2

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMTVKKIA IFGATGRTGL TTLAQAVQAG YEVTVLVRDS SRLPSEGPQP AHVVVGDVRQ AADVDKTVAG QEAVIVLLGT GNDLSPTTVM SEGTRNIVTA MKAHGVDKVV ACTSAFLLWD PTKVPPRLQD VTDDHIRMHK ILQESGLKYV AVMPPHIGDQ PLTGAYTVTL DGRGPSRVIS KHDLGHFMLR CLTTNEYDGH TTYPSHQYD