Recombinant muis CAMK2N1-eiwit

Camk2n1, also known as Calcium/calmodulin-dependent protein kinase II inhibitor 1, belongs to the CAMK2N family. This protein interacts with CAMK2B; the presence of Ca2+/calmodulin increases the interaction but is not essential. It also interacts with CAMK2A; this interaction requires CAMK2A activation by Ca2+. Camk2n1 is potent and specific inhibitor of CaM-kinase II (CAMK2) . Recombinant mouse Camk2n1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calcium/calmodulin-dependent protein kinase II inhibitor 1, Calcium/calmodulin-dependent protein kinase II inhibitor alpha, mCaMKIINalpha

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

10.9 kDa (101aa) Confirmed by MALDI-TOF

MCaMKIINalpha, Camk2n1, Calcium/calmodulin-dependent protein kinase II inhibitor alpha, Calcium/calmodulin-dependent protein kinase II inhibitor 1, AI89176, AI848599, 1810006K23Rik, ATGP3556-10 µg, ATGP3556-20 µg, ATGP3556-50 µg, ATGP3556-100 µg, ATGP3556-250 µg, ATGP3556-500 µg, ATGP3556-1 mg, ATGP3556-010, ATGP3556-020, ATGP3556-050, ATGP3556-100, ATGP3556-250, ATGP3556-500, ATGP3556-01M

Wang C., et al. (2008) J Biol Chem. 283 (17) :11565-74.; ; Meng F., et al. (2003) Brain Res. 967 (1-2) : 161-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Signal Transduction

NP_079727

Q6QWF9

Mouse

MSEVLPY GDEKLSPYGD GGDVGQIFSC RLQDTNNFFG AGQSKRPPKL GQIGRSKRVV IEDDRIDDVL KTMTDKAPPG V