Recombinant muis Cathepsine B-eiwit

CTSB, also known as cathepsin B preproprotein, is a papain-family cysteine protease that is normally located in lysosomes. It has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Recombinant mouse CTSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

Cathepsin B, Ctsb, CB, Cathepsin B1, CatB

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 2,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

36.4 kDa (330aa)

Cathepsin B, Cathepsin B, Ctsb, CB, ATGP3487-10 µg, ATGP3487-20 µg, ATGP3487-50 µg, ATGP3487-100 µg, ATGP3487-250 µg, ATGP3487-500 µg, ATGP3487-1 mg, ATGP3487-10, ATGP3487-20, ATGP3487-50, ATGP3487-100, ATGP3487-250, ATGP3487-500, ATGP3487-1

Podgorski I., et al. (2003) Biochem Soc Symp. 70:263-276.; ; Roshy S., et al. (2003) Cancer Metastasis Rev. 22:271-286.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_031824

P10605

Mouse

His18

HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH