Recombinant muis Cathepsine D-eiwit

Ctsd, also known as cathepsin D, is peptidase belonging to the family of aspartic peptidases. It is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. It plays an important role in alpha-synuclein degradation, and neuronal survival. It has also been shown to mediate apoptosis induced by various stimuli and p53-dependent tumor suppression. Recombinant mouse Ctsd, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 1,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

Cathepsin D, Ctsd, CatD, CD

Baculovirus

Mouse

His-Tag

Enzyme Activity, SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

44 kDa (398aa)

Cathepsin D, Cathepsin D, Ctsd, CatD, CD, ATGP3490-10 µg, ATGP3490-20 µg, ATGP3490-50 µg, ATGP3490-100 µg, ATGP3490-250 µg, ATGP3490-500 µg, ATGP3490-1 mg, ATGP3490-10, ATGP3490-20, ATGP3490-50, ATGP3490-100, ATGP3490-250, ATGP3490-500, ATGP3490-1

Crabtree D., et al. (2013) Neurosci Lett. 538:3-8.; ; Tsukuba T., et al. (2000) Mol Cells. 10:601-611.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_034113

P18242

Mouse

Ile21