Recombinant muis CD200 eiwit

CD200, also known as OX-2 membrane glycoprotein isoform 1, is one of the immunoglobulin superfamily. It plays an important role in anti-tumor immunity through interaction with its receptor, and overexpression of this protein effects on malignancies as well as on cancer stem cells. In T cells, this protein functions as a co-stimulatory effect that is independent of the CD28 pathway. It also plays an important role on myeloid cell regulation that is suggested by restricted expression of its receptor. Studies of this protein in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Recombinant mouse CD200, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse CD200 is coated at 1 µg/mL (100 ul/well) can bind CD200 R1 (CAT# ATGP3457) in a Functional ELISA assay.

Cd200, Mox2, OX2, OX-2 membrane glycoprotein isoform 1, MRC OX-2 antigen

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Mouse CD200 R1 (CAT# ATGP3457)

23.5 kDa (210aa)

, ATGP3964-10 µg, ATGP3964-20 µg, ATGP3964-50 µg, ATGP3964-100 µg, ATGP3964-250 µg, ATGP3964-500 µg, ATGP3964-1 mg, ATGP3964-10, ATGP3964-20, ATGP3964-50, ATGP3964-100, ATGP3964-250, ATGP3964-500, ATGP3964-1

Poongodi R., et al. (2018) Indian J Pathol Microbiol. 61:50-57. ; ; Kuwabara J., et al. (2018) Biochem Biophys Res Commun. 496:542-548.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_034948

O54901

Mouse

Gln31