Recombinant muis CD39L2/ENTPD6 eiwit

CD39L2, also known as ENTPD6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells. It is soluble monomeric enzyme, making it more amenable to thorough structural and fuctional analysis than the membrane-bound forms. Soluble CD39L2 preferentially exhibits nucleoside diphosphatase activity, which is depedent on the presence of divalent cations. It is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells. Also, it plays a role in the regulation and recruitment of platelet activation in heart. Recombinant mouse CD39L2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specific activity is > 80,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze GDP per minute at pH 7.5 at 25C.

Ectonucleoside triphosphate diphosphohydrolase 6 isoform1, 2700026H11Rik, Cd39l, Cd39l2, dJ738P15.3, NTPDa, NTPDase-6, Entpd6

HEK293

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Specific activity is > 80,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze GDP per minute at pH 7.5 at 25C.

47.3kDa (433aa)

, ATGP3984-10 µg, ATGP3984-20 µg, ATGP3984-50 µg, ATGP3984-100 µg, ATGP3984-250 µg, ATGP3984-500 µg, ATGP3984-1 mg, ATGP3984-10, ATGP3984-20, ATGP3984-50, ATGP3984-100, ATGP3984-250, ATGP3984-500, ATGP3984-1

Yeung G., et al, (2000) Biochemistry. 39:12916-12923.; ; Hicks-Berger CA, et al, (2000) J Biol Chem. 275:34041-34045.; ; Ivanenkov VV, et al, (2003) Biochemistry. 42:11726-11735.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Signal Transduction, Metabolism

NP_742115.2

Q3U0P5

Mouse

Asp

KWHRAS AAQAFFTIAG AASGARWTQQ AFSSPGSAAR GHEVFYGIMF DAGSTGTRIH VFQFARPPGE TPTLTHETFK ALKPGLSAYA DDVEKSAQGI QELLNVAKQH IPYDFWKATP LVLKATAGLR LLPGEKAQKL LQKVKEVFKA SPFLVGDDCV SIMNGTDEGV SAWITVNFLT GSLKTPGSSS VGMLDLGGGS TQITFLPRVE GTLQASPPGH LTALQMFNRT YKLYSYSYLG LGLMSARLAI LGGVEGKPAE NDKELVSPCL SPRFRGEWEH AEVTYRISGQ KAVGLYELCA SRVSEVLRNK VHRTEEAQHV DFYAFSYYYD LAASFGLIDA EKGGSLVVGD FEIAAKYVCR TLETQPPSSP FACMDLTYIS LLLHEFGFPG DKVLKLARKI DNVETSWALG AIFHYIDSLK RQKVPAL