Recombinant muis Ces2e eiwit

CES2E, also known as pyrethroid hydrolase Ces2e, is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it is widely distributed in cells especially in mammalian liver. It is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Recombinant mouse CES2E, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 30 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.

9030624L02Rik, Ces5, Ces2e

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 30unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.

60.5 kDa (541aa)

Ces2e, 9030624L02Rik, Ces5, Ces2e, ATGP3273-10 µg, ATGP3273-20 µg, ATGP3273-50 µg, ATGP3273-100 µg, ATGP3273-250 µg, ATGP3273-500 µg, ATGP3273-1 mg, ATGP3273-10, ATGP3273-20, ATGP3273-50, ATGP3273-100, ATGP3273-250, ATGP3273-500, ATGP3273-1

Stok JE., et al. (2004) J Biol Chem. 279:29863-29869.; ; Holmes RS., et al. (2008) Comp Biochem Physiol Part D Genomics Proteomics. 3:196-204.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_766347.1

Q8BK48

Mouse