Recombinant muis CHST5 eiwit

CHST5, also known as Carbohydrate sulfotransferase 5, is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. This sulfotransferase utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. It also mediates sulfation of keratan in cornea. It acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. It may also have activity toward O-linked sugars of mucin-type acceptors. There is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6. Recombinant mouse CHST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

Chst5, Carbohydrate sulfotransferase 5, Galactose, N-acetylglucosamine, N-acetylglucosamine 6-O-sulfotransferase 4, GST4, Intestinal N-acetylglucosamine-6-O-sulfotransferase, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, mIGn6ST, N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-3, Gn6st-3, I-GlcNAc-6-ST, I-GlcN, GST-4. GST-

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

42.9kDa (380aa)

, ATGP3995-10 µg, ATGP3995-20 µg, ATGP3995-50 µg, ATGP3995-100 µg, ATGP3995-250 µg, ATGP3995-500 µg, ATGP3995-1 mg, ATGP3995-10, ATGP3995-20, ATGP3995-50, ATGP3995-100, ATGP3995-250, ATGP3995-500, ATGP3995-1

Narentuya., et al, (2019) Sci Rep 9:4387.; ; Yasutaka Hayashida., et al, (2006) Proc Natl Acad Sci U S A 103:13333-13338.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Immunology

NP_064334

Q9QUP4

Mouse

Ala

SRQVP SSPAGLGERV HVLVLSSWRS GSSFVGQLFS QHPDVFYLME PAWHVWDTLS QGSAPALHMA VRDLIRSVFL CDMDVFDAYL PWRRNISDLF QWAVSRALCS PPVCEAFARG NISSEEVCKP LCATRPFGLA QEACSSYSHV VLKEVRFFNL QVLYPLLSDP ALNLRIVHLV RDPRAVLRSR EQTAKALARD NGIVLGTNGT WVEADPRLRV VNEVCRSHVR IAEAALHKPP PFLQDRYRLV RYEDLARDPL TVIRELYAFT GLGLTPQLQT WIHNITHGSG PGARREAFKT TSRDALSVSQ AWRHTLPFAK IRRVQELCGG ALQLLGYRSV HSELEQRDLS LDLLLPRGMD SFKWASSTEK QPES