Recombinant muis CRMP1-eiwit

Crmp1, also known as Collapsin response mediator protein 1, is necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. It plays a role in axon guidance, invasive growth and cell migration and may participate in cytokinesis. Recombinant mouse Crmp1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Collapsin response mediator protein 1 isoform 2, CRMP-1, Dpysl1, DRP-1, ULIP-3, ULip3, Dihydropyrimidinase-related protein 1, DRP-1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

64.8 kDa (597aa) confirmed by MALDI-TOF

Unc-33-like phosphoprotein 3, Unc 33 like phosphoprotein 3, ULIP-3, ULIP3, ULIP 3, Inactive dihydropyrimidinase, DRP-1, DRP1, DRP 1, Dpysl-1, Dpysl1, Dpysl 1, Dihydropyrimidinase-related protein 1, CRMP-1, CRMP1, CRMP 1, Collapsin response mediator protein 1 isoform 2, ATGP3744-10 µg, ATGP3744-20 µg, ATGP3744-50 µg, ATGP3744-100 µg, ATGP3744-250 µg, ATGP3744-500 µg, ATGP3744-1 mg, ATGP3744-010, ATGP3744-020, ATGP3744-050, ATGP3744-100, ATGP3744-250, ATGP3744-500, ATGP3744-01M

Shih JY., et al. (2001) J Natl cancer Inst. 93 (18) :1392-400.; ; Chen TC., et al. (2009) J Proteome Res. 8 (11) :4943-53.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.2M NaCl, 0.1mM PMSF

Neuroscience

NP_031791

P97427

Mouse

MGSSHHHHHH SSGLVPRGSH MGSEFMSHQG KKSIPHITSD RLLIRGGRII NDDQSFYADV YLEDGLIKQI GENLIVPGGV KTIEANGRMV IPGGIDVNTY LQKPSQGMTS ADDFFQGTKA ALAGGTTMII DHVVPEPGSS LLTSFEKWHE AADTKSCCDY SLHVDITSWY DGVREELEVL VQDKGVNSFQ VYMAYKDLYQ MSDSQLYEAF TFLKGLGAVI LVHAENGDLI AQEQKRILEM GITGPEGHAL SRPEELEAEA VFRAIAIAGR INCPVYITKV MSKSAADIIA LARKKGPLVF GEPIAASLGT DGTHYWSKNW AKAAAFVTSP PLSPDPTTPD YLTSLLACGD LQVTGSGHCP YSTAQKAVGK DNFTLIPEGV NGIEERMTVV WDKAVATGKM DENQFVAVTS TNAAKIFNLY PRKGRIAVGS DADVVIWDPD KMKTITAKSH KSTVEYNIFE GMECHGSPLV VISQGKIVFE DGNISVSKGM GRFIPRKPFP EHLYQRVRIR SKVFGLHSVS RGMYDGPVYE VPATPKHAAP APSAKSSPSK HQPPPIRNLH QSNFSLSGAQ IDDNNPRRTG HRIVAPPGGR SNITSLG