Recombinant muis Crystalline gamma D/CRYGD eiwit

Crygd, also known as gamma-crystalin D, belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in Crygd are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA) . Recombinant mouse Crygd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Gamma-crystallin D, Aey4, Cryg-1, DGcry-1, Lop12

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

23.5 kDa (197aa) Confirmed by MALDI-TOF

Crygd, Gamma-crystallin D, Aey4, Cryg-1, DGcry-1, Lop12, ATGP3670-10 µg, ATGP3670-20 µg, ATGP3670-50 µg, ATGP3670-100 µg, ATGP3670-250 µg, ATGP3670-500 µg, ATGP3670-1 mg, ATGP3670-10, ATGP3670-20, ATGP3670-50, ATGP3670-100, ATGP3670-250, ATGP3670-500, ATGP3670-1

Pande A., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (11) :6116-20; ; Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81 (1) :32-43

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Neuroscience

NP_031802

P04342

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMGKITFY EDRGFQGRHY ECSTDHSNLQ PYFSRCNSVR VDSGCWMLYE QPNFTGCQYF LRRGDYPDYQ QWMGFSDSVR SCRLIPHAGS HRIRLYEREE YRGQMIEFTE DCPSLQDRFH FNEIYSLNVL EGCWVLYDMT NYRGRQYLLR PGEYRRYHDW GAMNARVGSL RRVMDFY