Recombinant muis Cyclofiline A/PPIA-eiwit

PpiA also known as Peptidyl-prolyl cis-trans isomerase A. This is an enzyme found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but proline's unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Recombinant mouse ppiA, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, SP18

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.4 kDa (187aa) confirmed by MALDI-TOF

Cyclophilin A, Ppia, Peptidyl-prolyl cis-trans isomerase A, 2700098C05 Cphn, CyP-18, CypA, ATGP2981-10 µg, ATGP2981-20 µg, ATGP2981-50 µg, ATGP2981-100 µg, ATGP2981-250 µg, ATGP2981-500 µg, ATGP2981-1 mg, ATGP2981-10, ATGP2981-20, ATGP2981-50, ATGP2981-100, ATGP2981-250, ATGP2981-500, ATGP2981-1

Haendler B., et al. (1987) EMBO J. 6:947-950

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Cell Cycle

NP_032933

P17742

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMVNPTVF FDITADDEPL GRVSFELFAD KVPKTAENFR ALSTGEKGFG YKGSSFHRII PGFMCQGGDF TRHNGTGGRS IYGEKFEDEN FILKHTGPGI LSMANAGPNT NGSQFFICTA KTEWLDGKHV VFGKVKEGMN IVEAMERFGS RNGKTSKKIT ISDCGQL