Recombinant muis Cyclophiline 40/PID eiwit

Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin

Peptidyl-prolyl cis-trans isomerase D, Peptidylprolyl isomerase D, Cyclophilin D, Cyclophilin 40, CYP-40, PPIase D, Rotamase D

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin

43.4 kDa (395aa) confirmed by MALDI-TOF

Cyclophilin40, Ppid, Peptidyl-prolyl cis-trans isomerase D, 4930564J03Rik, CYP-40, Ppid1, Ppif, ATGP3724-10 µg, ATGP3724-20 µg, ATGP3724-50 µg, ATGP3724-100 µg, ATGP3724-250 µg, ATGP3724-500 µg, ATGP3724-1 mg, ATGP3724-10, ATGP3724-20, ATGP3724-50, ATGP3724-100, ATGP3724-250, ATGP3724-500, ATGP3724-1

Yokoi H., et al. (1996) Genomics. 1;35 (3) :448-55. ; ; Tavecchio M., et al. (2013) J. Biol. Chem. 22;288 (8) :5553-61.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Cell Cycle

NP_080628

Q9CR16

Mouse

MSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA