Recombinant muis Cystatine C-eiwit

CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy. Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

CST3, CysC

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

14.2 kDa (126aa) confirmed by MALDI-TOF

Cystatin C, CST3, CysC, ATGP3569-10 µg, ATGP3569-20 µg, ATGP3569-50 µg, ATGP3569-100 µg, ATGP3569-250 µg, ATGP3569-500 µg, ATGP3569-1 mg, ATGP3569-10, ATGP3569-20, ATGP3569-50, ATGP3569-100, ATGP3569-250, ATGP3569-500, ATGP3569-1

Janowski. R., et al. (2001) Nat. Struct. Biol. 8 (4) :316-20.; ; Mussap M., et al. (2004) Crit Rev Clin Lab Sci. 41 (5-6) :467-550.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_034106

P21460

Mouse

Ala21