Recombinant muis DDT-eiwit

Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5, 6-dihyroxyindole (DHI) . This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

D-dopachrome decarboxylase, C78655

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.5 kDa (141aa) confirmed by MALDI-TOF

DDT, D-dopachrome decarboxylase, C78655, ATGP3750-10 µg, ATGP3750-20 µg, ATGP3750-50 µg, ATGP3750-100 µg, ATGP3750-250 µg, ATGP3750-500 µg, ATGP3750-1 mg, ATGP3750-10, ATGP3750-20, ATGP3750-50, ATGP3750-100, ATGP3750-250, ATGP3750-500, ATGP3750-1

Hiyoshi M., et al. (2009) Toxicology. 255 (1-2) : 6-14.; ; Sugimoto H., et al., (1999) Biochemistry. 38 (11) :3268-79.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_034157

O35215

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L