Recombinant muis Dihydrofolaatreductase/DHFR-eiwit

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Dihydrofolate reductase, EC 1.5.1.3

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

23.8 kDa (207aa) confirmed by MALDI-TOF

DHFR, Dihydrofolate reductase, EC 1.5.1.3, ATGP0912-10 µg, ATGP0912-20 µg, ATGP0912-50 µg, ATGP0912-100 µg, ATGP0912-250 µg, ATGP0912-500 µg, ATGP0912-1 mg, ATGP0912-10, ATGP0912-20, ATGP0912-50, ATGP0912-100, ATGP0912-250, ATGP0912-500, ATGP0912-1

Chen MJ., et al. (1984) J Biol Chem. 259 (6) :3933-43. ; ; Cody V., et al. (2009) Biochemistry. 48 (8) :1702-11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Redox Proteins

NP_034179.1

P00375

Mouse

MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD