Recombinant muis DLL4 eiwit

DLL4, also known as delta-like protein 4, is involved in the Notch signaling pathway as Notch ligand. Therefore it negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, it inhibits V2a interneuron fate. Recombinant mouse DLL4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Dll4, Delta4

Baculovirus

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

55.8 kDa (512aa)

DLL4, Dll4, Delta4, ATGP3187-10 µg, ATGP3187-20 µg, ATGP3187-50 µg, ATGP3187-100 µg, ATGP3187-250 µg, ATGP3187-500 µg, ATGP3187-1 mg, ATGP3187-10, ATGP3187-20, ATGP3187-50, ATGP3187-100, ATGP3187-250, ATGP3187-500, ATGP3187-1

Luo H., et al. (2011) Proc Natl Acad Sci U S A. 109:E553-E562.; ; John R. Shutter., et al. (2000) Genes Dev. 14:1313-1318.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_062327

Q9JI71

Mouse