Recombinant muis EFG-eiwit

Egf, also known as Pro-epidermal growth factor isoform1, is a 8. 6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. Egf is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, Egf has been shown to inhibit gastric secretion, and to be involved in wound healing. Egf signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse Egf protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography

Mouse EGF in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/mL.

Epidermal growth factor, Pro-epidermal growth factor isoform1, AI790464

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/mL.

8.6 kDa (77aa) Confirmed by MALDI-TOF

EGF, Pro-epidermal growth factor isoform1, AI790464, ATGP3729-10 µg, ATGP3729-20 µg, ATGP3729-50 µg, ATGP3729-100 µg, ATGP3729-250 µg, ATGP3729-500 µg, ATGP3729-1 mg, ATGP3729-10, ATGP3729-20, ATGP3729-50, ATGP3729-100, ATGP3729-250, ATGP3729-500, ATGP3729-1, ATGP1532

Riese., et al (1998) Bioessays. 20 (1) :41-8.; ; Cohen S (1983) Cancer. 51 (10) :1787-91.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol

Hormones & Cytokines

NP_034243

P01132

Mouse

NSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR