Recombinant muis eIF-4E/EIF4E eiwit

Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, eIF-4F 25 kDa subunit

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

27.6 kDa (241aa) confirmed by MALDI-TOF

Eif4e, Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, ATGP3550-10 µg, ATGP3550-20 µg, ATGP3550-50 µg, ATGP3550-100 µg, ATGP3550-250 µg, ATGP3550-500 µg, ATGP3550-1 mg, ATGP3550-10, ATGP3550-20, ATGP3550-50, ATGP3550-100, ATGP3550-250, ATGP3550-500, ATGP3550-1

Piron F., et al. (2010) PLoS One. 5 (6) :e11313.; ; Cao R., et al. (2015) Nat neurosci. 18 (6) :855-62.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_031943.3

P63073

Mouse

MATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V