Recombinant muis Elongin B-eiwit

Elob, also known as elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimeric consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C) . It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant mouse Elob, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Elongin-B, EloB, Elongin 18 kDa subunit, RNA polymerase II transcription factor SIII subunit B, SIII p18, Transcription elongation factor B polypeptide 2, Tceb2

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.6 kDa (141aa) confirmed by MALDI-TOF

Elob, Elongin B, 0610040H15Rik, Tceb2, ATGP3814-10 µg, ATGP3814-20 µg, ATGP3814-50 µg, ATGP3814-100 µg, ATGP3814-250 µg, ATGP3814-500 µg, ATGP3814-1 mg, ATGP3814-10, ATGP3814-20, ATGP3814-50, ATGP3814-100, ATGP3814-250, ATGP3814-500, ATGP3814-1

Piessevaux J., et al. (2008) J Biol Chem. 283 (31) :21334-46.; ; Duan DR., et al. (1995) Science. 269 (5229) :1402-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 10% glycerol

Cell Cycle

NP_080581

P62869

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q