Recombinant muis Endoglin/CD105 eiwit

Eng, also known as endoglin, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. It is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. Recombinant mouse Eng, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse Endoglin (CAT# ATGP3361) is coated at 10 µg/mL (100 ul/well) can bind Human TGF-beta RI (CAT# ATGP4134) in a Functional ELISA assay.

AI528660, AI662476, CD105, Cell surface MJ7/18 antigen, Endo, Endoglin, Eng, ORW, ORW1, Osler-Rendu-Weber syndrome 1, S-endoglin

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human TGF-beta RI (CAT# ATGP4134) .

60.9 kDa (563aa)

AI528660, AI662476, CD 105, CD105, CD-105, Cell surface MJ7/18 antigen, Endo, Endoglin, Eng, ORW, ORW 1, ORW1, Osler Rendu Weber syndrome 1, Osler-Rendu-Weber syndrome 1, S endoglin, S-endoglin, ATGP3361-10 µg, ATGP3361-20 µg, ATGP3361-50 µg, ATGP3361-100 µg, ATGP3361-250 µg, ATGP3361-500 µg, ATGP3361-1 mg, ATGP3361-010, ATGP3361-020, ATGP3361-050, ATGP3361-100, ATGP3361-250, ATGP3361-500, ATGP3361-01M

Ten Dijke P., et al. (2008) Angiogenesis. 11:79-89.; ; Bernabeu C., et al. (2007) J Cell Biochem. 102:1375-1388.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_031958

Q63961

Mouse

Glu27